Advances in bioscience research usually arise as a result of the continu ing refinement of existing technologies. However, there are a number of occa sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac tion. This technique, first reported in 1985 by MuUis and his colleagues, pro vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

PCR Sequencing Protocols (Methods in Molecular Biology)

Ralph Rapley

Reference

Humana Press

<p>This handbook offers a collection of detailed descriptions of 50 major laboratory techniques utilized throughout molecular biology and biochemistry (including both nucleic acid and protein methods). The multitude of techniques are described in detail with a comprehensive background outlining the technique followed by a discussion of the application of the technique in the lab.</p>

Molecular Biomethods Handbook

Science & Math

Biological Sciences

Humana Press Inc.,U.S.

<p>...illuminates the core bioanalytical techniques used in laboratories throughout the world...written by highly- experienced scientists.</p>

Medical Biomethods Handbook

John M.

Ralph Rapley Eds Walker

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Books by Ralph Rapley