This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells―in one handy resource. 
This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series―each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Culture of Human Stem Cells

Science & Math

Freshney, R. Ian

Stacey, Glyn N.

Auerbach, Jonathan M.

Biological Sciences

Wiley-Liss

This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture.Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.
This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:
<ul>
<li>An all-new section addressing the additional caution required once a primary culture is converted to a cell line</li>
<li>Differentiation in stem cells and reprogramming of adult cells to form stem cells</li>
<li>A new section and protocol on cryopreservation by vitrification</li>
<li>Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes</li>
<li>A new section on organotypic culture</li>
<li>Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination</li>
<li>A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers</li>
</ul>
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians. Q&amp;A with Author R. Ian Freshney, PhD. Author R. Ian Freshney, PhD. R. Ian Freshney, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology at the University of Glasgow. He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells (now in its 6th Edition), and Culture of Human Stem Cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams. What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition? I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book. What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure. What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated. What advice would you offer to scientists writing or editing their first book? - Determine the precise niche that you wish to fill. - Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands. - Ensure the text is well illustrated with tables and diagrams. - Make sure the book is properly cross-referenced. - Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not. How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions. Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago? Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation. How do you envision the evolution of science writing over the next 5-10 years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online. What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo. What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Wiley/Blackwell

This immensely useful CD-ROM is an interactive, laboratory-based reference and instructional guide to techniques involved in the culture of animal cells. It provides a link between textual information and visual demonstrations of techniques in a manner that cannot be duplicated in any other medium. Special features include:  More than 50 step-by-step protocols for cell culture, including basic and specialized techniques, supported by detailed textual and background information. Overviews which summarize the protocols, link to text, video and supplemental material and facilitate navigation. Estimates are given for total procedure time and duration of each step. Extensive atlases of different cell types, culture stages, equipment photos, diagrams, tables, and graphs. Video sequences with narration that illustrate 16 basic cell culture techniques. Full search and print ability. A compendium of recipes for commonly used reagents A global directory of commercial suppliers Guidelines for setting up a cell culture laboratory Advice on laboratory safety and biohazards  Also available as a book & CD set: 0471332852 Minimum System Requirements PC  Windows(r) 3.1 and higher 486 processor 8 MB RAM 4 MB Hard Disk Space 4X CD-ROM recommended  Macintosh  System 7 8 MB RAM 4 MB Hard Disk Space 4X CD-ROM recommended

Freshney's Culture of Animal Cells: A Multimedia Guide

The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture. This crucial reference for cell culture techniques includes: * New Atlas of Cells section in full-color presentation * Extended coverage of molecular techniques, scale-up, and serum-free medium * New chapter on problem solving * Photographs of cell lines, contaminations, and equipment * Clear and concise tables and charts * Educated recommendations on safety issues, ethical consent, and ownership Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.

Culture of Animal Cells: A Manual of Basic Technique, 4th Edition

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: * An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols * New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering * An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment * Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text * Fully updated references * The clearest, most consistent presentation of step-by-step protocols available * Numerous diagrams, photographs, tables, and charts * Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

Culture of Animal Cells: A Manual of Basic Technique

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. 
New Topics covered include: <ul>
<li>the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting
</li> <li>cell interactions in cell culture</li> <li>new methods for separating cells</li> <li>new or refined methods for accessing cytotoxicity, viability, and mutagenicity</li> <li>experimental details for culture of specialized cells types not covered in previous editions</li> <li>new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy</li> </ul>
The revised and expanded third edition offers the following features: <ul>
<li>over 350 new reference to the primary literature</li> <li>an international list of cell banks</li> <li>an international listing of reagants and commercial supplies</li> <li>a subject index</li> <li>a glossary</li> </ul>
Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Culture of animal cells: A manual of basic technique

A.R. Liss

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

FRESHNEY’S CULTURE OF ANIMAL CELLS 
THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES 
Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. 
The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: \nProvides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field\nCovers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting\nFeatures a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation\nIncludes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print\nThe eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.\nFrom the Inside Flap\nTHE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES 
Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. 
The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: \nProvides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and e

Freshney's Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Capes-Davis, Amanda

See the best price to sell, buy, or rent books by Freshney, R. Ian. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

Books by Freshney, R. Ian

See the best price to sell, buy, or rent books by Freshney, R. Ian. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

Culture of Human Stem Cells

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Freshney's Culture of Animal Cells: A Multimedia Guide

Culture of Animal Cells: A Manual of Basic Technique, 4th Edition

Culture of Animal Cells: A Manual of Basic Technique

Culture of Animal Cells: A Manual of Basic Technique

Culture of animal cells: A manual of basic technique

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Freshney's Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications